Cardiac extracellular matrix proteomics: Challenges, techniques, and clinical implications
نویسندگان
چکیده
منابع مشابه
Editorial Extracellular Matrix Proteomics in Cardiac Ischemia / Reperfusion
Prolonged myocardial ischemia leads to myocyte necrosis, which initiates a localized inflammatory response that catabolizes the cellular debris and initiates deposition of extracellular matrix (ECM) proteins.1 In addition to providing a vital structural framework for the three-dimensional organization of cells, the ECM determines the physical properties of biological tissues and acts as a dynam...
متن کاملSystems biology—opportunities and challenges: the application of proteomics to study the cardiovascular extracellular matrix
Systems biology approaches including proteomics are becoming more widely used in cardiovascular research. In this review article, we focus on the application of proteomics to the cardiac extracellular matrix (ECM). ECM remodelling is a hallmark of many cardiovascular diseases. Proteomic techniques using mass spectrometry (MS) provide a platform for the comprehensive analysis of ECM proteins wit...
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ProteinChip technology, a suite of analytical tools that includes retentate chromatography, on-chip protein characterization, and multivariate analysis, allows researchers to examine patterns ofprotein expression and modification. Based on the surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) approach, ProteinChip technology has been pioneered by resea...
متن کاملExtracellular matrix proteomics in cardiac ischemia/reperfusion: the search is on.
Prolonged myocardial ischemia leads to myocytenecrosis, which initiates a localized inflammatory response that catabolizes the cellular debris and initiates deposition of extracellular matrix (ECM) proteins.1 In addition to providing a vital structural framework for the three-dimensional organization of cells, the ECM determines the physical properties of biological tissues and acts as a dynami...
متن کاملProteomics analysis of cardiac extracellular matrix remodeling in a porcine model of ischemia/reperfusion injury.
BACKGROUND After myocardial ischemia, extracellular matrix (ECM) deposition occurs at the site of the focal injury and at the border region. METHODS AND RESULTS We have applied a novel proteomic method for the analysis of ECM in cardiovascular tissues to a porcine model of ischemia/reperfusion injury. ECM proteins were sequentially extracted and identified by liquid chromatography tandem mass...
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ژورنال
عنوان ژورنال: PROTEOMICS - Clinical Applications
سال: 2015
ISSN: 1862-8346
DOI: 10.1002/prca.201500030